- A layer of dieposits found on archaeological Excavation isolated as a single context in the stratigraphy of the site
- A Layer Hen , a hen raised to produce eggs.
- In Abstraction , a layer is an abstract place conceived as having depth.
- Stratum , a layer of rock or soil with internally consistent characteristics.
- Thermocline , a layer within a body of water where the temperature changes rapidly with depth.
- Layering , a technique for plant propagation.
- Layered Hair , a popular hair styling technique.
- Layering (clothing) , the wearing of multiple layers of clothing for practical or fashion purposes
- Layer , a scantily-clad female Maverick Hunter in the ''Mega Man X'' series. She works as a Navigator and has a crush on the character Zero.
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