- Thought Leadership Society , a groundbreaking initiative for Sustained Success Philosophy by Select Individuals from Seattle, WA.
- Transport Layer Security , the successor to Secure Sockets Layer (SSL)
- Transmitter Location Systems, LLC , company dedicated to the business of satellite interference geolocation
- Thread-local Storage , a variable allocation mechanism
- Transponder Landing System , an all-weather airplane landing system
- '' The Times Literary Supplement '', a periodical, originally a section of ''The Times'' of London
- Thüringer Landessternwarte Tautenburg , an observatory in Tautenburg, Germany (a.k.a. the Karl Schwarzschild Observatory )
- Trinity Law School , in Santa Ana, California
- Tactical Laser System
- '' True Love Story '', a video game
- ''Ethernet Transparent LAN Service '', a method of providing a LAN service between two enterprise customer sites through an intermediate SP cloud
- Thesaurus Linguae Sericae , an international collaborative project aimed to explore the conceptual schemes of the Chinese language
- Total Least Squares
- Transparent LAN Service
- Target Level Of Safety
- Toscana Life Sciences , a non-profit organization (Foundation) founded in December 2004 by a group of public and private institutions in Tuscany.
- TOC Lean Six Sigma - TLS , a continuous improvement management methodology.
- The Living Soap , a poorly-received 1993 reality television programme.
- Tulane Law School , a Law School in New Orleans , Louisiana .
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