can refer to:
- Dr. William Worrall Mayo or one of his two sons, William James Mayo and Charles Horace Mayo
- Mayo Clinic , a world famous medical center in Rochester, Minnesota
- Mayo Medical School , a prestigous American medical school that is part of the Mayo Clinic and the Mayo College of Medicine
- Mayonnaise , sometimes abbreviated as ''mayo''
- Mayo College , a secondary educational institution in Ajmer, Rajasthan, India
- Mayo GAA , an inter county GAA team
- Mayo Language
- Mayo People , an Indigenous ethnic group who live in the states of Sinaloa and Sonora, Mexico
- Mayo (TV Series) , a TV series on BBC first broadcast in 2006
- County Mayo , a county in Ireland
- Division Of Mayo , an Australian Electoral Division in South Australia
- Mayo, County Mayo , a village in County Mayo, Ireland
- Mayo, Florida , a town in the U.S. state of Florida
- Mayo, Quebec , a town in Quebec, Canada
- Mayo River , a number of rivers called ''Mayo''
- Mayo, Trinidad And Tobago , a town in Trinidad and Tobago
- Mayo, Yukon , a town in Yukon, Canada
- Mayo, Maryland , a town in Maryland , USA
- Arthur Mayo (1840 - 1920), recipient of the Victoria Cross
- Ron Mayo (1986-present), KKTK Division Officer
- Barry Mayo , a radio executive
- Charles Herbert Mayo (1845 - 1929), an antiquarian
- Charles Horace Mayo (1865 - 1939), a physician and co-founder of the Mayo Clinic
- Elton Mayo (1880 - 1949), a psychologist and sociologist
- Helen Mayo (1878 - 1967), a women's health pioneer
- Iban Mayo (born 1977), a professional bicycle racer
- James Mayo, pen name of Stephen Coulter , (born 1913), English author
- James Mayo (born 1975), a singer-songwriter from Nashville, Tennessee
- Manolito Tolentino Mayo (1955 - 1983), a Philippine artist
- OJ Mayo (born 1987), an American basketball player
- Simon Mayo (born 1958), a disc jockey
- Steven Mayo (born 1960), the founder of Emissary International, a clinical research consulting company
- Virginia Mayo (1920 - 2005), an American film actress
- Whitman Mayo (1930 - 2001), an American television actor
- William Mayo, the vicar of Folke, Dorset, England c. 1845
- William Mayo (civil Engineer) (c. 1685 - 1744), the civil engineer who laid out the city of Richmond, Virginia
- William B. Mayo , (1860 - 1944), the chief power engineer of Ford Motor Company
- William James Mayo (1861 - 1939), co-founder of the Mayo Clinic
- William Leonidas Mayo (1861 - 1917), the founder and first president of Texas A&M University-Commerce (originally known as East Texas Normal College)
- William Worrall Mayo , co-founder of the Mayo Clinic
- Thomas Mayo Brewer (1814 - 1880), a American naturalist
- Antoine Malliarakis (aka Mayo), (1905-1990), a Greek-French painter and costume designer
- Richmond Mayo-Smith (1854 - 1901), an American economist
- Christopher Charles Mayo (born 1967), inventor of the goo film.
- Mayo Antonio D. Laus (born 1967), Operating Room RN
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