; Typographic characters
- Dot can be an abbreviation of the name Dorothy , which means "gift of god"
; People and fictional characters
- A cartoon character from ''Animaniacs'', one of Yakko, Wakko, And Dot
- Dot Branning , from ''EastEnders''
- Dot Matrix (ReBoot) , from the television series ''ReBoot''
- Dot Matrix (''Spaceballs'') , a character from the movie ''Spaceballs''
- Dot Moore , a Mobile, Alabama television personality
- Little Dot , published by Harvey Comics, a little girl obsessed with dots, spots and round, colorful objects
- Dot The Ant , an animated character from Disney Pixar's A Bug's Life
- Dot And Dash , the girl and the boy appearing in the PBS Kids idents
- Dot And The Kangaroo , children's book by Ethel C. Pedley, written in 1899
; Companies and agencies
; Other meanings
- DOT Language , the plain-text format for describing graphs used by Graphviz
- Dot Product , in mathematics
- Newton's Notation for differentiation, in mathematics and physics
- Dot (website)
- DNS Root Zone , the root domain (known as ".") of the Domain Name System
- Dictionary Of Occupational Titles (in the USA)
- Dorchester, Massachusetts
- Directly Observed Treatment , a way to administer medicine
- Dutch Open Telescope
- Damage Over Time , in computer and video games
- The Designated Order Turnaround system, in finance
- Diffuse Optical Tomography
- An element in Morse Code
- A song by Destiny's Child
- Gibson ES-335 , guitar, specifically referring to models with dot inlays
- --- Epiphone Dot , a budget copy of the ES-335
- Dot (album) , a single by the Los Angeles pop punk band ALL
- Tilaka , a dot worn on the forehead by Hindus
- Dot Matrix , a 2-dimensional array of dots used to generate characters, symbols and images
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