| Time-resolved Spectroscopy |
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TRANSIENT-ABSORPTION SPECTROSCOPY Transient-absorption spectroscopy is an extension of Absorption Spectroscopy . Here, the Absorbance at a particular Wavelength or range of wavelengths of a sample is measured as a function of time after Excitation by a flash of light. In a typical experiment, both the light for excitation ('pump') and the light for measuring the absorbance ('probe') are generated by a pulsed laser. If the process under study is slow, then the time resolution can be obtained with a continuous (i.e., not pulsed) probe beam and repeated conventional Spectrophotometric techniques. Examples of processes that can be studied:
OTHER MULTIPLE-PULSE TECHNIQUES Transient spectroscopy as discussed above is a technique that involves two pulses. There are many more techniques that employ two or more pulses, such as:
The interpretation of experimental data from these techniques is usually much more complicated than in transient-absorption spectroscopy. Nuclear Magnetic Resonance and Electron Spin Resonance are often implemented with multiple-pulse techniques, though with radio waves and micro waves instead of visible light. TIME-RESOLVED FLUORESCENCE SPECTROSCOPY Time-resolved fluorescence spectroscopy is an extension of Fluorescence Spectroscopy . Here, the Fluorescence of a sample is monitored as a function of time after excitation by a flash of light. The time resolution can be obtain in a number of ways, depending on the required sensitivity and time resolution:
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