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Rna Polymerase I




The process of transcription by Pol I is relatively unregulated ( RRNA for Ribosomes is always needed in large quantities). Correspondingly it undergoes a simple process of transcription, with few stages to be regulated.

In the process of Transcription (by any polymerase) there are three main stages:
#Initiation; requiring construction of the RNA polymerase complex on the gene's Promoter .
#Elongation; the writing of the RNA transcript.
#Termination; the finishing of RNA writing and dissasembly of the RNA polymerase complex.


RNAP I INITIATION

Initiation: the construction of the polymerase complex on the promoter.
RNAP I requires no TATA Box in the promoter, instead relying on an UCS (Upstream '''C'''ontrol '''S'''equence).

#UBF (Upstream '''B'''inding '''F'''actor) binds the UCS.
#UCS recruits and binds a protein complex incorporating TBP (TATA '''B'''inding '''P'''rotein) and three TAFs (TBP '''A'''ssociated '''F'''actors) called SL1. The TBP is forced to bind non-sequence specifically.
#Rrn3/TIFIA gets phosphorylated and binds RNAP I
#RNAP I binds to the UBF/SL1 complex via Rrn3/TIFIA, and transcription starts.