| Paper Chromatography |
Article Index for Paper |
Shopping Chromatography |
Website Links For Paper |
Information AboutPaper Chromatography |
| CATEGORIES ABOUT PAPER CHROMATOGRAPHY | |
| forensics | |
| chromatography | |
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Paper chromatography is an Analytical technique for separating and identifying compounds that are or can be colored, especially Pigment s. It is one of the frequently used forms of Chromatography . The Solvent moves up the Paper by Capillary Action , which occurs as a result of the attraction of the solvent molecules to the paper and to one another. As the solvent moves up the paper, it carries along any substance dissolved in it. The pigments are carried along at different rates because they are not equally soluble in the solvent and because they are attracted, to different degrees, to the Fiber s in the paper and through the formation of Intermolecular Bonds , such as Hydrogen Bond s. After development, the spots corresponding to different compounds may be located by their color, by Ultraviolet light, Ninhydrin or by treatment with Iodine vapors. As in most other forms of chromatography, paper chromatography uses Rf values to identify compounds. Rf values are calculated by dividing the farthest distance the pigment travels up the paper by the farthest distance the solvent travels (solvent front). Because Rf values are standard for a given compound, known Rf values can be used to identify a pigment in an experiment. |
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