| Northern Blot |
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| CATEGORIES ABOUT NORTHERN BLOT | |
| molecular biology | |
| laboratory techniques | |
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This technique was developed by James Alwine while working as a postdoc at Stanford University. A notable difference in the procedure (as compared with the Southern blot) is the addition of Formaldehyde in the agarose gel, which acts as a denaturant. As in the Southern blot, the hybridization probe may be made from DNA or RNA. A variant of the procedure known as the reverse northern blot was occasionally (although, infrequently) used. In this procedure, the substrate nucleic acid (that is affixed to the membrane) is a collection of isolated DNA fragments, and the probe is RNA extracted from a tissue and radioactively labelled. The use of DNA Microarray s that have come into widespread use in the late 1990s and early 2000s is more akin to the reverse procedure, in that they involve the use of isolated DNA fragments affixed to a substrate, and hybridization with a probe made from cellular RNA. Thus the reverse procedure, though originally uncommon, enabled the one-at-a-time study of gene expression using Northern analysis to evolve into Gene Expression Profiling , in which many (possibly all) of the genes in an organism may have their expression monitored. |
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