| Good's Buffers |
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Information AboutGood's Buffers |
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SELECTION CRITERIA Good sought to identify buffering compounds which met several criteria likely to be of value in biological research. # PKa . Because most biological reactions take place at near-neutral PH between 6 and 8, ideal buffers would have pKa values in this regime to provide maximum buffering capacity there. #Solubility. For ease in handling and because biological systems are in aqueous systems, good solubility in water was required. Low solubility in s and other cell compartments. #Membrane impermeability. Ideally, a buffer will not readily pass through cell membranes, this will also reduce the accumulation of buffer compound within Cell s. #Minimal salt effects. Highly ionic buffers may cause problems or complications in some biological systems. #Well-behaved cation interactions. If the buffers form Complex es with Cation ic Ligand s, the complexes formed should remain soluble. Ideally, at least some of the buffering compounds will not form complexes. #Stability. The buffers should be chemically stable, resisting Enzymatic and non-enzymatic degradation. #Optical absorbance. Buffers should not absorb visible or Ultraviolet light at wavelengths longer than 230 Nm so as not to interfere with commonly-used Spectrophotometric assays. #Ease of preparation. Buffers should be easily prepared and purified from inexpensive materials. GOOD'S BUFFERS The twelve buffers selected by Good are tabulated below. SEE ALSO REFERENCES
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