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VIRUS STRUCTURE AND CLASSIFICATION A major branch of virology is es, Fungal viruses, and Bacteriophage s (viruses infecting Bacteria , which include the most complex viruses). Another classification uses the geometrical shape of their Capsid (often a Helix or an Icosahedron ) or the virus' structure (e.g. presence or absence of a Lipid Envelope ). Viruses range in size from about 30 Nm to about 450 Nm , which means that most of them cannot be seen with Light Microscope s. The shape and structure of viruses can be studied with Electron Microscopy , with NMR Spectroscopy , and most importantly with X-ray Crystallography . The most useful and most widely used classification system distinguishes viruses according to the type of Nucleic Acid they use as genetic material and the Viral Replication method they employ to coax host cells into producing more viruses:
In addition virologists also study ''subviral particles'', infectious entities even smaller than viruses: Viroid s (naked circular RNA molecules infecting plants), Satellites (nucleic acid molecules with or without a capsid that require a helper virus for infection and reproduction), and Prion s ( Protein s that can exist in a conformation which induces other protein molecules to assume that same conformation). The latest report by the International Committee On Taxonomy Of Viruses (2005) lists 5450 viruses, organized in over 2,000 species, 287 genera, 73 families and 3 orders. The Taxa in virology are not necessarily Monophyletic . In fact, the evolutionary relationships of the various virus groups remain unclear, and three hypotheses regarding their origin exist: # Viruses arose from non-living matter, separately from and in parallel to other life forms, possibly in the form of self-reproducing RNA Ribozyme s similar to Viroid s. # Viruses arose from earlier, more competent cellular life forms that became parasites to host cells and subsequently lost most of their functionality; examples of such tiny parasitic prokaryotes are Mycoplasma and Nanoarchaea . # Viruses arose as parts of the genome of cells, most likely Transposon s or Plasmid s, that acquired the ability to "break free" from the host cell and infect other cells. It is of course possible that different alternatives apply to different virus groups. Of particular interest here is Mimivirus , a giant virus that infects Amoebae and carries much of the molecular machinery traditionally associated with bacteria. Is it a simplified version of a parasitic prokaryote, or did it originate as a simpler virus that acquired genes from its host? While viruses reproduce and evolve, they don't engage in Metabolism and depend on a host cell for reproduction. The often-debated question of whether they are alive or not is a matter of definition that does not affect the biological reality of viruses. MOLECULAR BIOLOGY RESEARCH AND VIRAL THERAPY Bacteriophage s, the viruses infecting Bacteria , can be relatively easily grown as Viral Plaque s on Bacterial Cultures . Bacteriophages occasionally move genetic material from one bacterial cell to another in a process known as Transduction , and this Horizontal Gene Transfer is one reason why they served as a major research tool in the early development of Molecular Biology . The Genetic Code , the function of Ribozyme s, the first Recombinant DNA and early Genetic Libraries were all arrived at using bacteriophages. Certain genetic elements derived from viruses, such as highly effective Promoter s, are commonly used in molecular biology research today. Growing animal viruses outside of the living host animal is more difficult. Classically, fertilized chicken eggs have often been used, but Cell Culture s are increasingly employed for this purpose today. Since viruses that infect Eukaryote s need to transport their genetic material into the host cell's Nucleus , they are attractive tools for introducing new genes into the host (known as Transformation or Transfection ), and this approach of using viruses as gene vectors is being pursued in the Gene Therapy of genetic diseases. An obvious problem to be overcome in viral gene therapy is the rejection of the transforming virus by the immune system. Oncolytic Virus es are viruses that preferably infect Cancer cells. While early efforts to employ these viruses in the therapy of cancer failed, there have been reports in 2005 and 2006 of encouraging preliminary results. {Link without Title} OTHER USES OF VIRUSES A new application of genetically engineered viruses in Nanotechnology was recently described; see Virus#Materials_science_and_nanotechnology . HISTORY A very early form of vaccination known as Variolation was developed several thousand years ago in China. It involved the application of materials from smallpox sufferers in order to immunize others. In 1796 Edward Jenner used cowpox to successfully immunize a young boy against smallpox, and this practice was widely adopted. Vaccinations against other viral diseases followed, including the successful Rabies vaccination by Louis Pasteur in 1886. The nature of viruses however was not clear to these researchers. In 1892 Dimitri Ivanovski showed that a disease of Tobacco Plants could be transmitted by extracts that were passed through filters fine enough to exclude even the smallest known bacteria. In 1898 Martinus Beijerinck , also working on tobacco plants, found that this "filterable agent" grew in the host and was thus not a mere Toxin . The question of whether the agent was a "living fluid" or a particle was however still open. In 1903 it was suggested for the first time that transduction by viruses might cause cancer. Such an oncovirus in chickens was described by Francis Peyton Rous in 1911; it was later called Rous Sarcoma Virus 1 and understood to be a retrovirus. Several other cancer-causing retroviruses have since been described. The existence of viruses that infect bacteria was first recognized by in 1952 showed that only DNA and not protein enters a bacterial cell upon infection with Bacteriophage T2 . Transduction of bacteria by bacteriophages was first described in the same year. While plant viruses and bacteriophages can be grown comparatively easily, animal viruses normally require a living host animal, which complicates their study immensely. In 1931 it was shown that Influenza Virus could be grown in fertilized chicken eggs, a method that is still used today to produce vaccines. In 1937, Max Theiler managed to grow the yellow fever virus in chicken eggs and produced a vaccine from an attenuated virus strain; this vaccine saved millions of lives and is still being used today. In 1949 John F. Enders , Thomas Weller and Frederick Robbins reported that they had been able to grow Poliovirus in cultured human embryonal cells, the first significant example of an animal virus grown outside of animals and chicken eggs. This work aided Jonas Salk in deriving a polio vaccine from killed polio viruses; this vaccine was shown to be effective in 1955. The first virus which could be Crystal ized and whose structure could therefore be studied in detail was Tobacco Mosaic Virus . Wendell Stanley achieved its crystallization in 1935, and clear X-ray diffraction pictures were obtained by Bernal and Fankuchen in 1941. Based on such pictures, Rosalind Franklin proposed the full structure of the tobacco mosaic virus in 1955. In 1963, the Hepatitis B Virus was discovered by Baruch Blumberg who went on to in 1963; he went on to construct a vaccine against Hepatitis B. In 1965, , the key enzyme that retroviruses use to translate their RNA into DNA, was first described in 1970, independently by Howard Temin and David Baltimore . The first retrovirus infecting humans was identified by Robert Gallo in 1974. Later it was found that reverse transcriptase is not specific to retroviruses; Retrotransposon s which code for reverse transcriptase are abundant in the genomes of all eukaryotes. About 10-40% of the human genome derives from such retrotransposons. In 1975 the functioning of oncoviruses was clarified considerably. Until that time, it was thought that these viruses carried certain genes called Oncogene s which, when inserted into the host's genome, would cause cancer. Michael Bishop and Harold Varmus showed that the oncogene of Rous sarcoma virus is in fact not specific to the virus but is contained in healthy animals of many species. The oncovirus can switch this pre-existing benign proto-oncogene on, turning it into a true oncogene. 1976 saw the first recorded outbreak of Ebola Hemorrhagic Fever , a highly lethal virally transmitted disease. In 1977, Frederick Sanger achieved the first complete sequencing of the Genome of any organism, a bacteriophage. In the same year, Richard Roberts and Phillip Sharp independently showed that the genes of Adenovirus contain Intron s and therefore require Gene Splicing . It was later realized that almost all genes of eukaryotes have introns as well. A world-wide vaccination campaign lead by the UN World Health Organization lead to the eradication of smallpox in 1979. In 1982, Stanley Prusiner discovered Prion s and showed that they cause Scrapie . The first cases of AIDS were reported in 1981, and HIV , the retrovirus causing it, was identified in 1983 by Robert Gallo and Luc Montagnier . Tests detecting HIV infection by detecting the presence of HIV antibody were developed. Subsequent tremendous research efforts turned HIV into the best studied virus. Human Herpes Virus 8 , the cause of Kaposi's Sarcoma which is often seen in AIDS patients, was identified in 1994. Several anti-retroviral drugs were developed in the late 1990s, decreasing AIDS mortality dramatically in developed countries. The first attempt at Gene Therapy in humans was performed in 1990; it involved a retrovirus and was unsuccessful. The giant Mimivirus , in some sense an intermediate between tiny prokaryotes and ordinary viruses, was described in 2003 and Sequenced in 2004. SEE ALSO EXTERNAL LINKS AND SOURCES
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