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Recombinant Dna Technology





USES

Recombinant DNA is used for Genetic Transformation to produce genetically modified organisms. Some examples of recombinant DNA products are Peptide Hormone Medication s including Insulin , Growth Hormone , and Oxytocin . Vaccine s can also be produced using recombinant processes. The organism most commonly used is '' Escherichia Coli ''.


PLASMIDS AND RECOMBINANT DNA TECHNOLOGY

Plasmids are extranuclear fragments of DNA present in some bacteria. Plasmids can transfer genetic material to other bacteria, allowing them to express the transmitted genes. Restriction enzymes which cut sequences of DNA at certain spots are used to splice into a plasmid the DNA sequence for the desired gene. The plasmid is then inserted into a bacterium in order to express the gene and produce the protein coded for by the gene. Large amounts of the protein can be produced in a factory with vats of the genetically engineered bacteria.

Eukaryotes cannot use circular DNA such as that present in a plasmid. Other systems, such as transfection with viruses, are used instead.


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