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Haematoxylin




Haematoxylin, '''hematoxylin''', '''Natural Black 1''', or '''C.I. 75290''' is extracted from the wood of the Logwood tree. When Oxidised it forms '''haematein''', a compound with rich blue-purple colour, and is used, together with a suitable Mordant (most commonly Fe(III) or Al(III) salts), to Stain cell nuclei prior to examination under a Microscope . Structures that stain with haematoxylin are called Basophilic .

Haematoxylin And Eosin Stain is one of the most commonly used stains in Histology .

Other common stain is Phosphotungstic Acid Haematoxylin , a mix of haematoxylin with Phosphotungstic Acid .

In 1970's, due to clear felling of forests in (CI 51050), Gallocyanin (CI 51030), Gallein (CI 45445) and Solochrome Cyanin (CI 43820). All four used Fe(III) as the mordant.


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