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Amplified Fragment Length Polymorphism
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AFLP-PCR is a highly sensitive method for detecting Polymorphism s in DNA .The technique was originally described by Zabeau & Vos in 1993. The procedure of this technique is divided into three steps:

#Digestion of total cellular DNA with one or more restriction enzymes and ligation of restriction half-site specific adaptors to all restriction fragments.
#Selective amplification of some of these fragments with two PCR primers that have corresponding adaptor and restriction site specific sequences.
#Electrophoretic separation of amplicons on a gel matrix, followed by visualisation of the band pattern.

Online programs for simulation of AFLP-PCR

ALFIE - BProkaryotes or uploaded sequences

In silico AFLP-PCR for prokaryotes ,some eukaryotes or uploaded sequences

A variation on AFLP is TE Display, used to detect transposable element mobility.


REFERENCES

  • Zabeau, M and P. Vos. 1993. Selective restriction fragment amplification: a general method for DNA fingerprinting. European Pattent Office, publication 0 534 858 A1, bulletin 93/13.

  • Van den Broeck et al., 1998

  • Casa et al., 2000

  • Biedler et al., 2003