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A1 Broth
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Typical formula (g/l)


Tryptone 20.0
Lactose 5.0
Sodium Chloride 5.0
Triton X-100 1.0
Salicin 0.5


Directions


Suspend the dry ingredients in one liter of cold distilled water. Gently heat until completely dissolved and distribute 9 ml into test tubes with an inverted Durham tube. Sterilize in an Autoclave at 121°C for 15 minutes. If needed, prepare multi-strength broth weighing the appropriate quantity of the dry medium. The final PH is 6.9 ± 0.1.