- RSS (file Format) , Really Simple Syndication, a form of web syndication used by news websites and weblogs
- Rashtriya Swayamsevak Sangh , a Hindu social service group in India
- Radio Service Software , a proprietary suite of programs sold by Motorola
- Regional Spatial Strategy provides a regional level planning framework for the regions of England
- Repetitive Strain Injury , also ''Repetitive stress syndrome'', an overuse syndrome
- Rochester Subway , which had the AAR reporting mark, RSS
- Rockdale, Sandow And Southern Railroad , which currently has the AAR reporting mark ''RSS''
- Royal Statistical Society , the main professional body for statisticians in the UK
- Residual Sum Of Squares , in regression models
- Resident Set Size
- Religious And Social Studies , a subject offered in many secondary schools in the United Kingdom
- Recruiting Sub-Station , a satellite office of a main military recruiting station.
- Rotating Service Structure , used in the Space Shuttle Program launches
- Reduced Space Symbology , a type of barcode developed by the Uniform Code Council
- Rural Sociological Society
- Recombination Signal Sequences , in immunological genetics, direct somatic recombination of gene segments
- Relay Spam Stopper , a Spam Blacklist run by Mail-abuse.org {Link without Title} , operated by MAPS (owned by Trend Micro).
- Resident Segment Size, a measurement of RAM usage. Represents actual physical RAM being used.
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