Murashige And Skoog Medium Website Links For
Medium 		 

Information About

Murashige And Skoog Medium
  CATEGORIES ABOUT MURASHIGE AND SKOOG MEDIUM
   
laboratory techniques
   
botany
   
growth media
   
microbiology
   
APPAREL
BABY
BEAUTY
BOOKS
CAR TOYS
CELL PHONES
DVD'S
ELECTRONICS
GOURMET FOOD
GROCERIES
HEALTH & PERSONAL
HOME & GARDEN
JEWELRY
MUSIC
MUSIC INSTRUMENTS
OFFICE PRODUCTS
SOFTWARE
SPORTING GOODS
TOOLS & HARDWARE
TOYS
VIDEO GAMES
SHOPPING HOME
MORE SHOPPING...



Murashige and Skoog medium or ('''''MSO '''''or ''''' MS0''''' ''(MS-zero)'') is a Plant Growth Medium used in the laboratories for cultivation of plant Cell Culture . MSO was invented by plant scientists Toshio Murashige and Folke K Skoog during Murashige's search for a new Plant Growth Regulator .

Macronutrients (mg/l)

Ammonium nitrate (NH4NO3) 1,650mg/l

Boric Acid (H3BO3) 6.2mg/l

  • H2O) 440mg/l


  • 6H2O) 0.025mg/l


  • 7H2O) 370mg/l


  • 5H2O) 0.025mg/l


Potassium phosphate (KH2PO4) 170mg/l

  • 7H2O) 27.8mg/l


Potassium nitrate (KNO3) 1,900mg/l

  • 4H2O) 22.3mg/l


Potassiom Iodine (KI) 0.83mg/l

  • 2H2O) 0.25mg/l


  • 7H2O) 8.6mg/l


  • 2H2Oa 37.2mg/lb


Common Organic Additives
i-Inositol 100mg/l

Nicotinic Acid 0.5mg/l

  • HCl 0.5mg/l


  • HCl 0.1mg/l


IAA 1-30mg/l

Kinetin 0.04-10mg/l

Glycine (recrytallized) 2.0g/l

Edamine 1.0g/l

Sucrose 20g/l

Agar 10g/l

It contains all nutrients that a plant needs to grow, as well as some (such as Iodine ) that are probably not Essential .

This medium has undergone extensive modifications by different growers to meet specific needs. Common variations include 1/2 MSO (half strength, often
used for Arabidopsis ).

The original citation for the published formulation is:

Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 18: 100-127.