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DEFINITION OF TERMS Resolving power or '''minimum resolvable distance''' is the ability of the optical components of a Microscope or Telescope to measure the angular separation of the points in the object that is being viewed. The term ''' Resolution ''' is the minimum distance between distinguishable Object s in an image, although the term is loosely used by many users of microscopes and telescopes to describe resolving power. In scientific analysis the term Resolution is generally used to describe the Precision with which any instrument measures and records (in an image or spectrum) any Variable in the specimen or sample under study. EXPLANATION The resolving power of a Lens is ultimately limited by Diffraction . The lens' Aperture is a "hole" that is analogous to a two-dimensional version of the single-slit Experiment ; Light passing through it Interferes with itself, creating a ring-shaped Diffraction pattern, known as the Airy Pattern , that blurs the image. An Empirical Diffraction limit is given by the Rayleigh criterion invented by Lord Rayleigh : The factor 1.22 is derived from a calculation of the position of the first dark ring surrounding the central Airy Disc of the Diffraction pattern. The calculation involves a Bessel Function and 1.22 is approximately the first positive zero of the Bessel function of the first kind, of order one divided by Pi . This factor is used to approximate the ability of the human Eye to distinguish two separate point sources depending on the Overlap of their Airy discs. Modern Telescope s and Microscope s with video Sensors may be slightly better than the human eye in their ability to discern overlap of Airy discs. Thus it is worth bearing in mind that the Rayleigh criterion is an empirical estimate of Resolution based on the assumption of a human observer, and may slightly underestimate the resolving power of a particular optical train. For specialized imaging, foreknowledge of some characteristics of the image can also improve on technical resolution limits through computerized Image Processing . For an ideal lens of Focal Length ''f'', the Rayleigh criterion yields a minimum spatial resolution, Δ''l'': :. This is the size of smallest object that the lens can resolve, and also the Radius of the smallest spot that a Collimated beam of Light can be focused to. The size is proportional to wavelength, ''λ'', and thus, for example, Blue light can be focused to a smaller spot than Red light. If the lens is focusing a beam of Light with a finite extent (e.g., a Laser beam), the value of ''D'' corresponds to the Diameter of the light beam, not the lens. Since the spatial resolution is inversely proportional to ''D'', this leads to the slightly surprising result that a wide beam of light may be focussed to a smaller spot than a narrow one. SINGLE TELESCOPE CASE Point-like sources separated by an Angle smaller than the angular resolution cannot be resolved. A single optical telescope may have an angular resolution less than one Arcsecond , but Astronomical Seeing and other atmospheric effects make attaining this very hard. The angular resolution ''R'' of a telescope can usually be approximated by : where λ :and ''D'' is the diameter of the telescope. The resulting ''R'' is in Radian s. Sources larger than the angular resolution are called extended sources or diffuse sources, and smaller sources are called point sources. For example, in the case of yellow light with a wavelength of 580 nm, for a resolution of 1 arc second, we need D = 12 cm. TELESCOPE ARRAY CASE The highest angular resolutions can be achieved by arrays of telescopes called , a large number of telescopes are required laid out in a 2 dimensional arrangement. The angular resolution ''R'' of an interferometer array can usually be approximated by : where λ :and ''B'' is the maximum separation of the telescopes in the array. The resulting ''R'' is in Radian s. Sources larger than the angular resolution are called extended sources or diffuse sources, and smaller sources are called point sources. For example, in order to form an image in yellow light with a wavelength of 580 nm, for a resolution of 1 milli-arcsecond, we need telescopes laid out in an array which is 120 m 120 m. MICROSCOPE CASE The resolution ''R'' depends on the Angular Aperture α: :. Here ''α'' is the ''collecting angle'' of the lens, which depends on the width of Objective Lens and its distance from the specimen. ''n'' is the '' Refractive Index '' of the medium in which the lens operates. ''λ'' is the wavelength of light illuminating or emanating from (in the case of fluorescence microscopy) the sample. Due to the limitations of the values ''α'', ''λ'', and ''n'', the resolution limit of a light microscope using es (''n'' = 1.56): : SEE ALSO |
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